› 2ifg

X-ray diffraction
3.4Å resolution

Structure of the extracellular segment of human TRKA in complex with nerve growth factor

Released:
DOI: 10.2210/pdb2ifg/pdb
PDB 2ifg coloured by chain and viewed from the front.
PDB 2ifg coloured by chain and viewed from the side.
PDB 2ifg coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors.
Wehrman T, He X, Raab B, Dukipatti A, Blau H, Garcia KC
Neuron 53 25-38 (2007)
PMID: 17196528

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
High affinity nerve growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04629 (Residues: 36-382; Coverage: 45%)
Gene names: MTC, NTRK1, TRK, TRKA
Structure domains:
Beta-nerve growth factor Chains: E, F
Molecule details ›
Chains: E, F
Length: 120 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01138 (Residues: 122-241; Coverage: 54%)
Gene names: NGF, NGFB
Sequence domains: Nerve growth factor family
Structure domains: Cystine-knot cytokines

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, NDG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, NDG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 110.215Å b: 81.681Å c: 115.748Å
α: 90° β: 104.15° γ: 90°
R-values:
R R work R free
0.297 0.297 0.331
Expression system: Spodoptera frugiperda

Quick links

Citations

38 review citations

Nerve Growth Factor Signaling and Its Contribution to Pain.
Barker et al. (2020)
37 more

13 mentions without citation

Discovery of Molecular Therapeutics for Glaucoma: Challenges, Successes, and Promising Directions.
Donegan et al. (2016)
12 more