PDBe 2idk

X-ray diffraction
2.55Å resolution

Crystal Structure of Rat Glycine N-Methyltransferase Complexed With Folate

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.46 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P13255 (Residues: 2-293; Coverage: 100%)
Gene name: Gnmt
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 57.427Å b: 84.751Å c: 132.245Å
α: 90° β: 91.61° γ: 90°
R-values:
R R work R free
0.24 0.24 0.298
Expression system: Escherichia coli BL21(DE3)