› › Function and Biology

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position and an abasic site analog at the target base partner position

Source organism: Thermus aquaticus
Biochemical function: site-specific DNA-methyltransferase (adenine-specific) activity
Biological process: methylation
Cellular component: not assigned

Quick links

EC 2.1.1.72: Site-specific DNA-methyltransferase (adenine-specific)

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Systematic name:
S-adenosyl-L-methionine:adenine in DNA N(6)-methyltransferase
Alternative Name(s):
  • Modification methylase
  • N-6 adenine-specific DNA methylase
  • Restriction-modification system

GO terms

Sequence families

Pfam Protein families (Pfam)
PF12950
Domain description: TaqI-like C-terminal specificity domain
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of Pfam domain PF12950 (TaqI-like C-terminal specificity domain) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].

PF07669
Domain description: Eco57I restriction-modification methylase
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of Pfam domain PF07669 (Eco57I restriction-modification methylase) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].

InterPro InterPro annotations
IPR029063
Domain description: S-adenosyl-L-methionine-dependent methyltransferase superfamily
Occurring in:
  1. Type II methyltransferase M.TaqI
IPR025931
Domain description: TaqI-like C-terminal specificity domain
Occurring in:
  1. Type II methyltransferase M.TaqI
IPR023135
Domain description: N6 adenine-specific DNA methyltransferase, TaqI class, C-terminal
Occurring in:
  1. Type II methyltransferase M.TaqI
IPR002052
Domain description: DNA methylase, N-6 adenine-specific, conserved site
Occurring in:
  1. Type II methyltransferase M.TaqI
IPR011639
Domain description: Type II methyltransferase M.TaqI-like domain
Occurring in:
  1. Type II methyltransferase M.TaqI
IPR021188
Domain description: N6 adenine-specific DNA methyltransferase, TaqI class
Occurring in:
  1. Type II methyltransferase M.TaqI

Structure domains

CATH CATH domains
3.90.220.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Adenine-n6-DNA-methyltransferase TaqI; Chain A, domain 2
Homology: Adenine-n6-DNA-methyltransferase Taqi, Chain A, domain 2
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of CATH domain 3.90.220.10 (Adenine-n6-DNA-methyltransferase TaqI; Chain A, domain 2) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].
3.40.50.150
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Vaccinia Virus protein VP39
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of CATH domain 3.40.50.150 (Rossmann fold) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].
SCOP SCOP annotations
116735
Class: Alpha and beta proteins (a+b)
Fold: DNA methylase specificity domain
Superfamily: DNA methylase specificity domain
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of SCOP domain 116735 (TaqI C-terminal domain-like) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].
88787
Class: Alpha and beta proteins (a/b)
Fold: S-adenosyl-L-methionine-dependent methyltransferases
Superfamily: S-adenosyl-L-methionine-dependent methyltransferases
Occurring in:
  1. Type II methyltransferase M.TaqI
The deposited structure of PDB entry 2ibt contains 2 copies of SCOP domain 88787 (DNA methylase TaqI, N-terminal domain) in Type II methyltransferase M.TaqI. Showing 1 copy in chain E [auth A].

Quick links