2ibt

X-ray diffraction
1.7Å resolution

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position and an abasic site analog at the target base partner position

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type II methyltransferase M.TaqI Chains: A, D
Molecule details ›
Chains: A, D
Length: 421 amino acids
Theoretical weight: 47.93 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14385 (Residues: 1-421; Coverage: 100%)
Gene name: taqIM
Sequence domains:
Structure domains:
5'-D(*GP*TP*TP*CP*GP*(2PR)P*TP*GP*TP*C)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
5'-D(*GP*AP*CP*AP*(3DR)P*CP*GP*(6MA)P*AP*C)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 10 nucleotides
Theoretical weight: 2.93 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 59.339Å b: 68.699Å c: 114.022Å
α: 90° β: 92.09° γ: 90°
R-values:
R R work R free
0.169 0.167 0.203
Expression systems:
  • Escherichia coli
  • Not provided