› 2ibm

X-ray diffraction
3.2Å resolution

A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA

Released:
DOI: 10.2210/pdb2ibm/pdb
PDB entry 2ibm coloured by chain, front view.
PDB entry 2ibm coloured by chain, side view.
PDB entry 2ibm coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA.
Zimmer J, Li W, Rapoport TA
J Mol Biol 364 259-65 (2006)
PMID: 16989859

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA Chains: A, B
Molecule details ›
Chains: A, B
Length: 780 amino acids
Theoretical weight: 88.92 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28366 (Residues: 1-780; Coverage: 93%)
Gene names: BSU35300, div+, secA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, NSLS BEAMLINE X25
Spacegroup: I212121
Unit cell:
a: 125.834Å b: 166.833Å c: 211.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.321 0.321 0.323
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

Protein translocation across the bacterial cytoplasmic membrane.
Driessen et al. (2008)
21 more

11 mentions without citation

The Sec System: Protein Export in Escherichia coli.
Crane et al. (2017)
10 more