X-ray diffraction
2.55Å resolution

Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+

Source organism: Homo sapiens
Entry authors: Kavanagh KL, Papagrigoriou E, Salah E, Lukacik P, Smee C, Burgess N, von Delft F, Weigelt J, Arrowsmith C, Sundstrom M, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-hydroxyisobutyrate dehydrogenase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 33.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P31937 (Residues: 40-336; Coverage: 88%)
Gene name: HIBADH
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 49.627Å b: 167.319Å c: 71.089Å
α: 90° β: 107.8° γ: 90°
R R work R free
0.176 0.174 0.22
Expression system: Escherichia coli BL21(DE3)