› 2i4j

X-ray diffraction
2.1Å resolution

Crystal structure of the complex between PPARgamma and the agonist LT160 (ureidofibrate derivative)

Released:
DOI: 10.2210/pdb2i4j/pdb
PDB 2i4j coloured by chain and viewed from the front.
PDB 2i4j coloured by chain and viewed from the side.
PDB 2i4j coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Insights into the mechanism of partial agonism: crystal structures of the peroxisome proliferator-activated receptor gamma ligand-binding domain in the complex with two enantiomeric ligands.
Pochetti G, Godio C, Mitro N, Caruso D, Galmozzi A, Scurati S, Loiodice F, Fracchiolla G, Tortorella P, Laghezza A, Lavecchia A, Novellino E, Mazza F, Crestani M
J. Biol. Chem. 282 17314-24 (2007)
PMID: 17403688

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 223-504; Coverage: 56%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand DRJ 1 x DRJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 93.142Å b: 60.947Å c: 118.106Å
α: 90° β: 103.26° γ: 90°
R-values:
R R work R free
0.25 0.247 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structural Biology-Based Exploration of Subtype-Selective Agonists for Peroxisome Proliferator-Activated Receptors.
Miyachi H. (2021)
5 more

7 mentions without citation

Metabolism of the Endocannabinoid Anandamide: Open Questions after 25 Years.
Maccarrone M. (2017)
6 more