› 2i3g

X-ray diffraction
1.85Å resolution

Crystal structure of N-Acetyl-gamma-Glutamyl-Phosphate Reductase (Rv1652) from Mycobacterium tuberculosis in complex with NADP+.

Released:
DOI: 10.2210/pdb2i3g/pdb
PDB 2i3g coloured by chain and viewed from the front.
PDB 2i3g coloured by chain and viewed from the side.
PDB 2i3g coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of N-acetyl-gamma-glutamyl-phosphate reductase from Mycobacterium tuberculosis in complex with NADP(+).
Cherney LT, Cherney MM, Garen CR, Niu C, Moradian F, James MN
J. Mol. Biol. 367 1357-69 (2007)
PMID: 17316682

Function and Biology Details

Reaction catalysed: 
N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-L-glutamyl 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyl-gamma-glutamyl-phosphate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 36.34 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPZ9 (Residues: 1-352; Coverage: 100%)
Gene names: MTCY06H11.17, Rv1652, argC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 141.456Å b: 78.214Å c: 88.034Å
α: 90° β: 127.46° γ: 90°
R-values:
R R work R free
0.163 0.161 0.205
Expression system: Escherichia coli

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Citations

1 review citation

Structural genomics approach to drug discovery for Mycobacterium tuberculosis.
Ioerger et al. (2009)
4 other articles

3 mentions without citation

A unified statistical model to support local sequence order independent similarity searching for ligand-binding sites and its application to genome-based drug discovery.
Xie et al. (2009)
2 more