PDBe 2i32

X-ray diffraction
2.7Å resolution

Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone chaperone ASF1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y294 (Residues: 1-157; Coverage: 77%)
Gene names: ASF1A, CGI-98, HSPC146
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like
Protein HIRA Chains: E, F
Molecule details ›
Chains: E, F
Length: 57 amino acids
Theoretical weight: 6.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54198 (Residues: 425-472; Coverage: 5%)
Gene names: DGCR1, HIR, HIRA, TUPLE1
Sequence domains: HIRA B motif

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P6522
Unit cell:
a: 116.226Å b: 116.226Å c: 167.599Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.269
Expression system: Escherichia coli