› 2i2a

X-ray diffraction
2.1Å resolution

Crystal structure of LmNADK1 from Listeria monocytogenes

Released:
DOI: 10.2210/pdb2i2a/pdb
PDB entry 2i2a coloured by chain, front view.
PDB entry 2i2a coloured by chain, side view.
PDB entry 2i2a coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
NAD kinases use substrate-assisted catalysis for specific recognition of NAD.
Poncet-Montange G, Assairi L, Arold S, Pochet S, Labesse G
J Biol Chem 282 33925-34 (2007)
PMID: 17686780

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 31.05 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y8D7 (Residues: 1-264; Coverage: 100%)
Gene names: lmo0968, nadK1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I222
Unit cell:
a: 61.902Å b: 77.429Å c: 117.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.261
Expression system: Escherichia coli

Quick links

Citations

6 review citations

NADPH-generating systems in bacteria and archaea.
Spaans et al. (2015)
5 more

3 mentions without citation

Toward an accurate prediction of inter-residue distances in proteins using 2D recursive neural networks.
Kukic et al. (2014)
2 more