2i0z

X-ray diffraction
1.84Å resolution

Crystal structure of a FAD binding protein from Bacillus cereus, a putative NAD(FAD)-utilizing dehydrogenases

Released:
Source organism: Bacillus cereus
Entry authors: Minasov G, Shuvalova L, Vorontsov II, Kiryukhina O, Abdullah J, Collart FR, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(FAD)-utilizing dehydrogenases Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.01 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q816V9 (Residues: 1-423; Coverage: 100%)
Gene name: BC_4706
Sequence domains: HI0933-like protein
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 46.716Å b: 92.366Å c: 97.504Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.248
Expression system: Escherichia coli BL21(DE3)