2hsw

X-ray diffraction
1.99Å resolution

Crystal structure of the uridine phosphorylase from Salmonella typhimurium in unliganded state at 1.99A resolution

Released:
Entry authors: Timofeev VI, Dontsova MV, Gabdoulkhakov AG, Mikhailov AM

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.17 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1F6 (Residues: 1-253; Coverage: 100%)
Gene names: STM3968, STMD1.21, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: R3
Unit cell:
a: 151.66Å b: 151.66Å c: 47.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Escherichia coli BL21(DE3)