› 2ho1

X-ray diffraction
2Å resolution

Functional Characterization of Pseudomonas Aeruginosa pilF

Released:
DOI: 10.2210/pdb2ho1/pdb
PDB entry 2ho1 coloured by chain, front view.
PDB entry 2ho1 coloured by chain, side view.
PDB entry 2ho1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa Type IV pilus secretin.
Koo J, Tammam S, Ku SY, Sampaleanu LM, Burrows LL, Howell PL
J Bacteriol 190 6961-9 (2008)
PMID: 18776008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV pilus assembly protein PilF Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 29 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXJ2 (Residues: 11-252; Coverage: 100%)
Gene names: PA3805, pilF
Sequence domains:
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 137.099Å b: 69.047Å c: 70.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.209 0.248
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
13 more

9 mentions without citation

Bacterial secretins: Mechanisms of assembly and membrane targeting.
Silva et al. (2020)
8 more