2hl3

X-ray diffraction
2.03Å resolution

Crystal structure of the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued) in complex with human EB1 C-terminal hexapeptide

Released:
Source organism: Homo sapiens

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynactin subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14203 (Residues: 18-111; Coverage: 7%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain
Microtubule-associated protein RP/EB family member 1 Chain: C
Molecule details ›
Chain: C
Length: 6 amino acids
Theoretical weight: 826 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15691 (Residues: 263-268; Coverage: 2%)
Gene name: MAPRE1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P212121
Unit cell:
a: 43.062Å b: 55.068Å c: 66.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.223
Expression systems:
  • Escherichia coli
  • Not provided