› 2hk2

X-ray diffraction
2.3Å resolution

Crystal structure of mevalonate diphosphate decarboxylase from Staphylococcus aureus (monoclinic form)

Released:
DOI: 10.2210/pdb2hk2/pdb
PDB entry 2hk2 coloured by chain, front view.
PDB entry 2hk2 coloured by chain, side view.
PDB entry 2hk2 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of Trypanosoma brucei and Staphylococcus aureus mevalonate diphosphate decarboxylase inform on the determinants of specificity and reactivity.
Byres E, Alphey MS, Smith TK, Hunter WN
J Mol Biol 371 540-53 (2007)
PMID: 17583736

Function and Biology Details

Reaction catalysed: 
ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diphosphomevalonate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 37.38 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FD84 (Residues: 1-327; Coverage: 100%)
Gene names: G6Y24_14745, H2639_10340, M1K003_2052, NCTC7878_01231, NCTC7972_01342, QU38_09965, SAMEA70153168_02225, mvaD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 85.495Å b: 53.061Å c: 100.882Å
α: 90° β: 94.82° γ: 90°
R-values:
R R work R free
0.194 0.191 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
2 more

3 mentions without citation

Discovery of a metabolic alternative to the classical mevalonate pathway.
Dellas et al. (2013)
2 more