X-ray diffraction
1.25Å resolution

Crystal structure of V224H design intermediate for GFP metal ion reporter

Source organism: Aequorea victoria
Entry authors: Barondeau DP, Tubbs JL, Tainer JA, Getzoff ED

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.92 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 51.63Å b: 62.15Å c: 69.136Å
α: 90° β: 90° γ: 90°
R R work R free
0.128 0.128 0.165
Expression system: Escherichia coli BL21(DE3)