› 2hjo

X-ray diffraction
1.25Å resolution

Crystal structure of V224H design intermediate for GFP metal ion reporter

Released:
DOI: 10.2210/pdb2hjo/pdb
PDB 2hjo coloured by chain and viewed from the front.
PDB 2hjo coloured by chain and viewed from the side.
PDB 2hjo coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Entry authors: Barondeau DP, Tubbs JL, Tainer JA, Getzoff ED

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.92 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 51.63Å b: 62.15Å c: 69.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.128 0.165
Expression system: Escherichia coli BL21(DE3)

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