2hhv

X-ray diffraction
1.55Å resolution

T:O6-methyl-guanine in the polymerase-2 basepair position

Released:
Primary publication:
The structural basis for the mutagenicity of O(6)-methyl-guanine lesions.
Proc. Natl. Acad. Sci. U.S.A. 103 19701-6 (2006)
PMID: 17179038

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5KWC1 (Residues: 300-878; Coverage: 66%)
Gene names: GK2730, polA
Sequence domains: DNA polymerase family A
Structure domains:
5'-D(*GP*CP*GP*AP*TP*CP*AP*GP*CP*TP*TP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*GP*TP*AP*CP*(6OG)P*AP*GP*CP*TP*GP*AP*TP*CP*GP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 86.956Å b: 93.357Å c: 106.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.239
Expression systems:
  • Escherichia coli BL21
  • Not provided