› 2hhp

X-ray diffraction
1.8Å resolution

Structure of yeast poly(A) polymerase in a closed conformation.

Released:
DOI: 10.2210/pdb2hhp/pdb
PDB entry 2hhp coloured by chain, front view.
PDB entry 2hhp coloured by chain, side view.
PDB entry 2hhp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
X-ray crystallographic and steady state fluorescence characterization of the protein dynamics of yeast polyadenylate polymerase.
Balbo PB, Toth J, Bohm A
J Mol Biol 366 1401-15 (2007)
PMID: 17223131

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase Chain: A
Molecule details ›
Chain: A
Length: 530 amino acids
Theoretical weight: 60.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P29468 (Residues: 1-530; Coverage: 93%)
Gene names: PAP1, YKR002W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 2 x FLC
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 58.483Å b: 84.918Å c: 67.721Å
α: 90° β: 111.78° γ: 90°
R-values:
R R work R free
0.198 0.197 0.236
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
3 more

8 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
7 more