› 2hgz

X-ray diffraction
2.5Å resolution

Crystal structure of a p-benzoyl-L-phenylalanyl-tRNA synthetase

Released:
DOI: 10.2210/pdb2hgz/pdb
PDB 2hgz coloured by chain and viewed from the front.
PDB 2hgz coloured by chain and viewed from the side.
PDB 2hgz coloured by chain and viewed from the top.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural basis for the recognition of para-benzoyl-L-phenylalanine by evolved aminoacyl-tRNA synthetases.
Liu W, Alfonta L, Mack AV, Schultz PG
Angew. Chem. Int. Ed. Engl. 46 6073-5 (2007)
PMID: 17628477

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.93 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 2-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PBF 1 x PBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 101.639Å b: 101.639Å c: 71.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.21 0.27
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Incorporation of Non-Canonical Amino Acids.
Leisle et al. (2015)
3 more

3 mentions without citation

Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids.
Klippenstein et al. (2014)
2 more