› 2hdf

X-ray diffraction
2.65Å resolution

Crystal structure of the Colicin I receptor Cir from E.coli

Released:
DOI: 10.2210/pdb2hdf/pdb
PDB 2hdf coloured by chain and viewed from the front.
PDB 2hdf coloured by chain and viewed from the side.
PDB 2hdf coloured by chain and viewed from the top.
Source organism: Escherichia coli
Entry authors: Buchanan SK, Esser L, Lukacik P

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin I receptor Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 71.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17315 (Residues: 26-663; Coverage: 100%)
Gene names: JW2142, b2155, cir, cirA, feuA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SR 1 x SR
Ligand OES 2 x OES
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 90.593Å b: 84.336Å c: 99.458Å
α: 90° β: 109.16° γ: 90°
R-values:
R R work R free
0.241 0.239 0.291
Expression system: Escherichia coli BL21(DE3)

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Citations

14 review citations

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
13 more

4 mentions without citation

Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.
Botos et al. (2016)
3 more