X-ray diffraction
2.23Å resolution

Crystal structure of PDE9 in complex with IBMX


Function and Biology Details

Reaction catalysed:
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 38.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: O76083 (Residues: 241-566; Coverage: 55%)
Gene name: PDE9A
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 103.513Å b: 103.513Å c: 269.727Å
α: 90° β: 90° γ: 90°
R R work R free
0.215 0.215 0.231
Expression system: Escherichia coli BL21