› 2hcb

X-ray diffraction
3.51Å resolution

Structure of AMPPCP-bound DnaA from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2hcb/pdb
PDB entry 2hcb coloured by chain, front view.
PDB entry 2hcb coloured by chain, side view.
PDB entry 2hcb coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structural basis for ATP-dependent DnaA assembly and replication-origin remodeling.
Erzberger JP, Mott ML, Berger JM
Nat Struct Mol Biol 13 676-83 (2006)
PMID: 16829961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosomal replication initiator protein DnaA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 37.73 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66659 (Residues: 77-399; Coverage: 81%)
Gene names: aq_322, dnaA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ACP 4 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 99.076Å b: 117.714Å c: 200.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.266 0.298
Expression system: Escherichia coli

Quick links

Citations

50 review citations

DNA replication initiation: mechanisms and regulation in bacteria.
Mott et al. (2007)
49 more

21 mentions without citation

The AAA+ superfamily of functionally diverse proteins.
Snider et al. (2008)
20 more