X-ray diffraction
1.58Å resolution

Crystal structure of HCV NS5B RNA polymerase in complex with novel class of dihydropyrone-containing inhibitor.


Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Capsid protein C Chain: A
Molecule details ›
Chain: A
Length: 576 amino acids
Theoretical weight: 64.27 KDa
Source organism: Hepacivirus C
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q99AU2 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P41212
Unit cell:
a: 83.255Å b: 83.255Å c: 179.83Å
α: 90° β: 90° γ: 90°
R R work R free
0.215 0.215 0.236
Expression system: Escherichia coli BL21(DE3)