› 2h9w

X-ray diffraction
1.82Å resolution

Green fluorescent protein ground states: the influence of a second protonation site near the chromophore

Released:
DOI: 10.2210/pdb2h9w/pdb
PDB 2h9w coloured by chain and viewed from the front.
PDB 2h9w coloured by chain and viewed from the side.
PDB 2h9w coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Green fluorescent protein ground states: the influence of a second protonation site near the chromophore.
Bizzarri R, Nifosì R, Abbruzzetti S, Rocchia W, Guidi S, Arosio D, Garau G, Campanini B, Grandi E, Ricci F, Viappiani C, Beltram F
Biochemistry 46 5494-504 (2007)
PMID: 17439158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.14 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-237; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
2 modified residues:
Ligand CRO 1 x CRO
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 51.886Å b: 62.933Å c: 71.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.186
Expression system: Escherichia coli

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Citations

5 review citations

Fluorescent proteins of the EosFP clade: intriguing marker tools with multiple photoactivation modes for advanced microscopy.
Nienhaus et al. (2021)
4 more