2h7y

X-ray diffraction
2.1Å resolution

Pikromycin Thioesterase with covalent affinity label

Released:

Function and Biology Details

Reactions catalysed:
Malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH = narbonolide + 7 CoA + 7 CO(2) + 5 NADP(+) + 2 H(2)O
Malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH = 10-deoxymethynolide + 6 CoA + 6 CO(2) + 5 NADP(+) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Narbonolide/10-deoxymethynolide synthase PikA4, module 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 33.79 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZGI2 (Residues: 1049-1346; Coverage: 22%)
Gene name: pikAIV
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 107.736Å b: 130.649Å c: 56.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Escherichia coli