2h7s

X-ray diffraction
2.15Å resolution

L244A mutant of Cytochrome P450cam

Released:

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, C
Molecule details ›
Chains: A, C
Length: 414 amino acids
Theoretical weight: 46.51 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 57.704Å b: 59.853Å c: 114.124Å
α: 90° β: 104.69° γ: 90°
R-values:
R R work R free
0.235 0.235 0.249
Expression system: Escherichia coli