› 2h5r

X-ray diffraction
1.6Å resolution

Crystal structure of mStrawberry at pH 10.5

Released:
DOI: 10.2210/pdb2h5r/pdb
PDB 2h5r coloured by chain and viewed from the front.
PDB 2h5r coloured by chain and viewed from the side.
PDB 2h5r coloured by chain and viewed from the top.
Source organism: Discosoma sp.
Primary publication:
Novel chromophores and buried charges control color in mFruits.
Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ
Biochemistry 45 9639-47 (2006)
PMID: 16893165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.68 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 7-221; Coverage: 96%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 48.646Å b: 44.177Å c: 61.179Å
α: 90° β: 105.98° γ: 90°
R-values:
R R work R free
0.166 0.166 0.217
Expression system: Escherichia coli

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Citations

37 review citations

Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to Study Dickeya and Pectobacterium Virulence and Biocontrol.
Bourigault et al. (2021)
36 more

1 mention without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)