› 2h5e

X-ray diffraction
2.8Å resolution

Crystal structure of E.coli polypeptide release factor RF3

Released:
DOI: 10.2210/pdb2h5e/pdb
PDB entry 2h5e coloured by chain, front view.
PDB entry 2h5e coloured by chain, side view.
PDB entry 2h5e coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.
Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H
Cell 129 929-41 (2007)
PMID: 17540173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide chain release factor RF3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 529 amino acids
Theoretical weight: 59.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7I4 (Residues: 1-529; Coverage: 100%)
Gene names: JW5873, b4375, miaD, prfC, tos
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 73.174Å b: 239.781Å c: 69.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.262
Expression system: Escherichia coli

Quick links

Citations

27 review citations

What recent ribosome structures have revealed about the mechanism of translation.
Schmeing et al. (2009)
26 more

7 mentions without citation

The evolutionary and functional diversity of classical and lesser-known cytoplasmic and organellar translational GTPases across the tree of life.
Atkinson GC. (2015)
6 more