› 2h3u

X-ray diffraction
1.9Å resolution

Crystal structure of murine carnitine acetyltransferase in complex with carnitine and CoA

Released:
DOI: 10.2210/pdb2h3u/pdb
PDB 2h3u coloured by chain and viewed from the front.
PDB 2h3u coloured by chain and viewed from the side.
PDB 2h3u coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
Crystal structures of murine carnitine acetyltransferase in ternary complexes with its substrates.
Hsiao YS, Jogl G, Tong L
J. Biol. Chem. 281 28480-7 (2006)
PMID: 16870616

Function and Biology Details

Reactions catalysed: 
Octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine
Acetyl-CoA + carnitine = CoA + O-acetylcarnitine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carnitine O-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 599 amino acids
Theoretical weight: 68.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47934 (Residues: 30-625; Coverage: 95%)
Gene name: Crat
Sequence domains: Choline/Carnitine o-acyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:
Ligand 152 2 x 152
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 164.51Å b: 89.74Å c: 122.95Å
α: 90° β: 128.96° γ: 90°
R-values:
R R work R free
0.193 0.193 0.235
Expression system: Escherichia coli

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Citations

2 review citations

Dioxygen and Metabolism; Dangerous Liaisons in Cardiac Function and Disease.
Angelini et al. (2017)
1 more

2 mentions without citation

Crystal structures of murine carnitine acetyltransferase in ternary complexes with its substrates.
Hsiao et al. (2006)
1 more