› 2h1f

X-ray diffraction
2.4Å resolution

E. coli heptosyltransferase WaaC with ADP

Released:
DOI: 10.2210/pdb2h1f/pdb
PDB entry 2h1f coloured by chain, front view.
PDB entry 2h1f coloured by chain, side view.
PDB entry 2h1f coloured by chain, top view.
Source organism: Escherichia coli O6
Primary publication:
Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose.
Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A
J Mol Biol 363 383-94 (2006)
PMID: 16963083

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipopolysaccharide heptosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.35 KDa
Source organism: Escherichia coli O6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2VC26 (Residues: 1-326; Coverage: 100%)
Gene names: c4447, rfaC
Sequence domains: Glycosyltransferase family 9 (heptosyltransferase)
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 78.86Å b: 88.86Å c: 89.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
4 more

4 mentions without citation

The Glycosyltransferases of LPS Core: A Review of Four Heptosyltransferase Enzymes in Context.
Cote et al. (2017)
3 more