X-ray diffraction
1.9Å resolution

Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH


Function and Biology Details

Reaction catalysed:
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Mono(ADP-ribosyl)transferase SpvB Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.62 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
  • Canonical: P21454 (Residues: 392-591; Coverage: 34%)
Gene names: mkaA, spvB
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

Cofactor: Ligand NAI 1 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3221
Unit cell:
a: 55.585Å b: 55.585Å c: 143.577Å
α: 90° β: 90° γ: 120°
R R work R free
0.196 0.194 0.235
Expression system: Escherichia coli