› › Function and Biology

Structure of a USP8-NRDP1 complex

Source organism: Homo sapiens
Biochemical function: ubiquitin protein ligase activity
Biological process: protein ubiquitination
Cellular component: not assigned

Quick links

EC 2.3.2.27: RING-type E3 ubiquitin transferase

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Systematic name:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein]-L-lysine ubiquitin transferase (isopeptide bond-forming; RING-type)
Alternative Name(s):
  • RING E3 ligase
  • Ubiquitin transferase RING E3

EC 3.4.19.12: Ubiquitinyl hydrolase 1

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Systematic name:
-
Alternative Name(s):
  • Ubiquitin C-terminal hydrolase
  • Ubiquitin carboxyl-terminal hydrolase
  • Ubiquitin thiolesterase
  • Yeast ubiquitin hydrolase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF08941
Domain description: USP8 interacting
Occurring in:
  1. E3 ubiquitin-protein ligase NRDP1
The deposited structure of PDB entry 2gwf contains 3 copies of Pfam domain PF08941 (USP8 interacting) in E3 ubiquitin-protein ligase NRDP1. Showing 1 copy in chain D.

PF00581
Domain description: Rhodanese-like domain
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8
The deposited structure of PDB entry 2gwf contains 3 copies of Pfam domain PF00581 (Rhodanese-like domain) in Ubiquitin carboxyl-terminal hydrolase 8. Showing 1 copy in chain E.

InterPro InterPro annotations
IPR001763
Domain description: Rhodanese-like domain
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8
IPR036873
Domain description: Rhodanese-like domain superfamily
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8
IPR037255
Domain description: NRDP1, C-terminal
Occurring in:
  1. E3 ubiquitin-protein ligase NRDP1
IPR015036
Domain description: E3 ubiquitin-protein ligase NRDP1
Occurring in:
  1. E3 ubiquitin-protein ligase NRDP1
IPR014729
Domain description: Rossmann-like alpha/beta/alpha sandwich fold
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8

Structure domain

CATH CATH domain
3.40.250.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Oxidized Rhodanese; domain 1
Homology: Rhodanese-like domain
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8
The deposited structure of PDB entry 2gwf contains 3 copies of CATH domain 3.40.250.10 (Oxidized Rhodanese; domain 1) in Ubiquitin carboxyl-terminal hydrolase 8. Showing 1 copy in chain E.
SCOP SCOP annotations
160089
Class: Alpha and beta proteins (a+b)
Fold: NRDP1 C-terminal domain-like
Superfamily: NRDP1 C-terminal domain-like
Occurring in:
  1. E3 ubiquitin-protein ligase NRDP1
The deposited structure of PDB entry 2gwf contains 3 copies of SCOP domain 160089 (USP8 interacting domain) in E3 ubiquitin-protein ligase NRDP1. Showing 1 copy in chain D.
117586
Class: Alpha and beta proteins (a/b)
Fold: Rhodanese/Cell cycle control phosphatase
Superfamily: Rhodanese/Cell cycle control phosphatase
Occurring in:
  1. Ubiquitin carboxyl-terminal hydrolase 8
The deposited structure of PDB entry 2gwf contains 3 copies of SCOP domain 117586 (Ubiquitin carboxyl-terminal hydrolase 8, USP8) in Ubiquitin carboxyl-terminal hydrolase 8. Showing 1 copy in chain E.

Quick links