› 2gw3

X-ray diffraction
1.4Å resolution

Crystal structure of stony coral fluorescent protein Kaede, green form

Released:
DOI: 10.2210/pdb2gw3/pdb
PDB 2gw3 coloured by chain and viewed from the front.
PDB 2gw3 coloured by chain and viewed from the side.
PDB 2gw3 coloured by chain and viewed from the top.
Source organism: Trachyphyllia geoffroyi
Primary publication:
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.
Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M
J. Mol. Biol. 372 918-26 (2007)
PMID: 17692334

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kaede Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.86 KDa
Source organism: Trachyphyllia geoffroyi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8I6J8 (Residues: 1-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
1 modified residue:
Ligand CR8 2 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 132.496Å b: 80.476Å c: 53.528Å
α: 90° β: 113.82° γ: 90°
R-values:
R R work R free
0.197 0.197 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

16 review citations

Fluorescent proteins of the EosFP clade: intriguing marker tools with multiple photoactivation modes for advanced microscopy.
Nienhaus et al. (2021)
15 more

5 mentions without citation

The structure of mAG, a monomeric mutant of the green fluorescent protein Azami-Green, reveals the structural basis of its stable green emission.
Ebisawa et al. (2010)
4 more