› 2gv8

X-ray diffraction
2.1Å resolution

Crystal structure of flavin-containing monooxygenase (FMO) from S.pombe and NADPH cofactor complex

Released:
DOI: 10.2210/pdb2gv8/pdb
PDB entry 2gv8 coloured by chain, front view.
PDB entry 2gv8 coloured by chain, side view.
PDB entry 2gv8 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Mechanism of action of a flavin-containing monooxygenase.
Eswaramoorthy S, Bonanno JB, Burley SK, Swaminathan S
Proc Natl Acad Sci U S A 103 9832-7 (2006)
PMID: 16777962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-specific monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 50.18 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HFE4 (Residues: 1-447; Coverage: 100%)
Gene names: SPBP16F5.08c, fmo1
Sequence domains:
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

3 bound ligands:
Ligand FAD 2 x FAD
Ligand NDP 2 x NDP
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 59.31Å b: 72.53Å c: 80.37Å
α: 98.59° β: 107.4° γ: 101.72°
R-values:
R R work R free
0.232 0.232 0.259
Expression system: Escherichia coli

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Citations

13 review citations

Flavoenzymes.
Joosten et al. (2007)
12 more

9 mentions without citation

Drug Discovery and Development of Novel Therapeutics for Inhibiting TMAO in Models of Atherosclerosis and Diabetes.
Steinke et al. (2020)
8 more