› 2gud

X-ray diffraction
0.94Å resolution

Crystal structure of a complex of griffithsin with mannose at 0.94 A resolution

Released:
DOI: 10.2210/pdb2gud/pdb
PDB entry 2gud coloured by chain, front view.
PDB entry 2gud coloured by chain, side view.
PDB entry 2gud coloured by chain, top view.
Source organism: Griffithsia
Primary publication:
Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding.
Ziółkowska NE, O'Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A
Structure 14 1127-35 (2006)
PMID: 16843894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Griffithsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 12.73 KDa
Source organism: Griffithsia
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: P84801 (Residues: 1-121; Coverage: 100%)
Sequence domains: Jacalin-like lectin domain
Structure domains: Jacalin-like lectin domain

Ligands and Environments

4 bound ligands:
Ligand MAN 6 x MAN
Ligand BMA 2 x BMA
Ligand EDO 1 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 53.74Å b: 39.44Å c: 57.16Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.136 0.136 0.156
Expression system: Nicotiana benthamiana

Quick links

Citations

25 review citations

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
24 more

17 mentions without citation

Griffithsin: An Antiviral Lectin with Outstanding Therapeutic Potential.
Lusvarghi et al. (2016)
16 more