2gtp

X-ray diffraction
2.55Å resolution

Crystal structure of the heterodimeric complex of human RGS1 and activated Gi alpha 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 37.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63096 (Residues: 32-354; Coverage: 91%)
Gene name: GNAI1
Structure domains:
Regulator of G-protein signaling 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 145 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08116 (Residues: 63-205; Coverage: 68%)
Gene names: 1R20, BL34, IER1, RGS1
Sequence domains: Regulator of G protein signaling domain
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 90.451Å b: 102.904Å c: 128.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.276
Expression system: Escherichia coli