2gqs

X-ray diffraction
2.05Å resolution

SAICAR Synthetase Complexed with CAIR-Mg2+ and ADP

Released:

Function and Biology Details

Reaction catalysed:
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7D7 (Residues: 1-237; Coverage: 100%)
Gene names: JW2461, b2476, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 59.43Å b: 67.12Å c: 149.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.263
Expression system: Escherichia coli