› 2gm8

X-ray diffraction
2.5Å resolution

TenA Homolog/Thi-4 Thiaminase complexed with product 4-amino-5-hydroxymethyl-2-methylpyrimidine

Released:
DOI: 10.2210/pdb2gm8/pdb
PDB entry 2gm8 coloured by chain, front view.
PDB entry 2gm8 coloured by chain, side view.
PDB entry 2gm8 coloured by chain, top view.
Source organism: Pyrobaculum aerophilum str. IM2
Entry authors: Sawaya MR, Chan S, Han GW, Perry LJ, Pashkov I

Function and Biology Details

Reaction catalysed: 
Thiamine + pyridine = 1-((4-amino-2-methylpyrimidin-5-yl)methyl)pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiaminase-2/PQQC domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 25.23 KDa
Source organism: Pyrobaculum aerophilum str. IM2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZZM9 (Residues: 2-212; Coverage: 100%)
Gene name: PAE0170
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

2 bound ligands:
Ligand HMH 4 x HMH
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 70.647Å b: 94.287Å c: 73.198Å
α: 90° β: 113.65° γ: 90°
R-values:
R R work R free
0.189 0.187 0.237
Expression system: Escherichia coli

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