EC 4.6.1.16: tRNA-intron lyase
Reaction catalysed:
PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Systematic name:
PretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming)
Alternative Name(s):
- Splicing endonuclease
- Transfer ribonucleate intron endoribonuclease
- Transfer splicing endonuclease
- tRNA splicing endonuclease
- tRNATRPintron endonuclease
GO terms
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence families
Protein families (Pfam)
![The deposited structure of PDB entry 2gjw contains 4 copies of Pfam domain PF01974 (tRNA intron endonuclease, catalytic C-terminal domain) in tRNA-splicing endonuclease. Showing 1 copy in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_Pfam_PF01974_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 4 copies of Pfam domain <span class='highlight'>PF01974 (tRNA intron endonuclease, catalytic C-terminal domain)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 1 copy in chain <span class='highlight'>G [auth A]</span>.")
![The deposited structure of PDB entry 2gjw contains 4 copies of Pfam domain PF02778 (tRNA intron endonuclease, N-terminal domain) in tRNA-splicing endonuclease. Showing 1 copy in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_Pfam_PF02778_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 4 copies of Pfam domain <span class='highlight'>PF02778 (tRNA intron endonuclease, N-terminal domain)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 1 copy in chain <span class='highlight'>G [auth A]</span>.")
InterPro annotations
IPR036167
Domain description:
tRNA intron endonuclease, catalytic domain-like superfamily
Occurring in:
Occurring in:
Structure domains
CATH domains
3.40.1350.10
Class:
Alpha Beta
Architecture:
3-Layer(aba) Sandwich
Topology:
Trna Endonuclease; Chain: A, domain 1
Homology:
Trna Endonuclease; Chain: A, domain 1
Occurring in:
![The deposited structure of PDB entry 2gjw contains 4 copies of CATH domain 3.40.1350.10 (Trna Endonuclease; Chain: A, domain 1) in tRNA-splicing endonuclease. Showing 1 copy in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_CATH_3.40.1350.10_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 4 copies of CATH domain <span class='highlight'>3.40.1350.10 (Trna Endonuclease; Chain: A, domain 1)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 1 copy in chain <span class='highlight'>G [auth A]</span>.")
3.40.1170.20
Class:
Alpha Beta
Architecture:
3-Layer(aba) Sandwich
Topology:
MutS, DNA mismatch repair protein, domain I
Homology:
tRNA intron endonuclease, N-terminal domain
Occurring in:
![The deposited structure of PDB entry 2gjw contains 4 copies of CATH domain 3.40.1170.20 (MutS, DNA mismatch repair protein, domain I) in tRNA-splicing endonuclease. Showing 1 copy in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_CATH_3.40.1170.20_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 4 copies of CATH domain <span class='highlight'>3.40.1170.20 (MutS, DNA mismatch repair protein, domain I)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 1 copy in chain <span class='highlight'>G [auth A]</span>.")
![The deposited structure of PDB entry 2gjw contains 4 copies of CATH domain 3.40.1350.150 (Trna Endonuclease; Chain: A, domain 1) in tRNA-splicing endonuclease. Showing 1 copy in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_CATH_3.40.1350.150_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 4 copies of CATH domain <span class='highlight'>3.40.1350.150 (Trna Endonuclease; Chain: A, domain 1)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 1 copy in chain <span class='highlight'>G [auth A]</span>.")
SCOP annotations
53033
Class:
Alpha and beta proteins (a/b)
Fold:
Restriction endonuclease-like
Superfamily:
tRNA-intron endonuclease catalytic domain-like
Occurring in:
![The deposited structure of PDB entry 2gjw contains 8 copies of SCOP domain 53033 (tRNA-intron endonuclease catalytic domain-like) in tRNA-splicing endonuclease. Showing 2 copies in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_SCOP_53033_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 8 copies of SCOP domain <span class='highlight'>53033 (tRNA-intron endonuclease catalytic domain-like)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 2 copies in chain <span class='highlight'>G [auth A]</span>.")
55268
Class:
Alpha and beta proteins (a+b)
Fold:
MutS N-terminal domain-like
Superfamily:
tRNA-intron endonuclease N-terminal domain-like
Occurring in:
![The deposited structure of PDB entry 2gjw contains 8 copies of SCOP domain 55268 (tRNA-intron endonuclease N-terminal domain-like) in tRNA-splicing endonuclease. Showing 2 copies in chain G [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2gjw_4_A_SCOP_55268_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2gjw</span> contains 8 copies of SCOP domain <span class='highlight'>55268 (tRNA-intron endonuclease N-terminal domain-like)</span> in <span class='highlight'>tRNA-splicing endonuclease</span>. Showing 2 copies in chain <span class='highlight'>G [auth A]</span>.")