› 2gi9

X-ray diffraction
1.14Å resolution

Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy

Released:
DOI: 10.2210/pdb2gi9/pdb
PDB entry 2gi9 coloured by chain, front view.
PDB entry 2gi9 coloured by chain, side view.
PDB entry 2gi9 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Backbone conformational constraints in a microcrystalline U-15N-labeled protein by 3D dipolar-shift solid-state NMR spectroscopy.
Franks WT, Wylie BJ, Stellfox SA, Rienstra CM
J Am Chem Soc 128 3154-5 (2006)
PMID: 16522090

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.23 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 25.197Å b: 36.384Å c: 50.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 not available 0.181
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Multidimensional solid state NMR of anisotropic interactions in peptides and proteins.
Wylie et al. (2008)
2 more

19 mentions without citation

Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy.
Zhou et al. (2007)
18 more