› 2ghp

X-ray diffraction
2.7Å resolution

Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24

Released:
DOI: 10.2210/pdb2ghp/pdb
PDB entry 2ghp coloured by chain, front view.
PDB entry 2ghp coloured by chain, side view.
PDB entry 2ghp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.
Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN, Butcher SE, Brow DA
J Mol Biol 367 1447-58 (2007)
PMID: 17320109

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U4/U6 snRNA-associated-splicing factor PRP24 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 292 amino acids
Theoretical weight: 33.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P49960 (Residues: 1-291; Coverage: 66%)
Gene names: PRP24, YM8156.10C, YMR268C
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 114.608Å b: 125.848Å c: 196.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.264
Expression system: Escherichia coli

Quick links

Citations

8 review citations

RNA recognition motifs: boring? Not quite.
Cléry et al. (2008)
7 more

7 mentions without citation

Detailed close-ups and the big picture of spliceosomes.
Jurica MS. (2008)
6 more