X-ray diffraction
1.7Å resolution

CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate

Source organism: Agrobacterium sp. CP4
Primary publication:
Molecular basis for the herbicide resistance of Roundup Ready crops.
Proc. Natl. Acad. Sci. U.S.A. 103 13010-5 (2006)
PMID: 16916934

Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 47.62 KDa
Source organism: Agrobacterium sp. CP4
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9R4E4 (Residues: 1-455; Coverage: 100%)
Gene name: aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 63.172Å b: 44.719Å c: 76.959Å
α: 90° β: 106.01° γ: 90°
R R work R free
0.148 0.148 0.174
Expression system: Escherichia coli BL21(DE3)