2gff

X-ray diffraction
1.75Å resolution

Crystal Structure of Yersinia pestis LsrG

Released:
Source organism: Yersinia pestis
Entry authors: de Carvalho-Kavanagh M, Schafer J, Lekin T, Toppani D, Chain P, Lao V, Motin V, Garcia E, Segelke B

Function and Biology Details

Reaction catalysed:
(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.47 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7CG46 (Residues: 1-96; Coverage: 100%)
Gene names: YPO0407, YP_3774, lsrG, y3774
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 56.174Å b: 56.174Å c: 119.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.258
Expression system: Escherichia coli BL21