2gf0

X-ray diffraction
1.9Å resolution

The crystal structure of the human DiRas1 GTPase in the inactive GDP bound state

Released:
Source organism: Homo sapiens
Entry authors: Turnbull AP, Papagrigoriou E, Yang X, Schoch G, Elkins J, Gileadi O, Salah E, Bray J, Wen-Hwa L, Fedorov O, Niesen FE, von Delft F, Weigelt J, Edwards A, Arrowsmith C, Sundstrom M, Doyle D, Structural Genomics Consortium (SGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Di-Ras1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95057 (Residues: 1-198; Coverage: 100%)
Gene names: DIRAS1, GBTS1, RIG
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 64.053Å b: 94.031Å c: 61.214Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.161 0.16 0.208
Expression system: Escherichia coli