X-ray diffraction
2.6Å resolution

Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP-Man


Function and Biology Details

Reaction catalysed:
GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol = GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphatidyl-myo-inositol mannosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
  • Canonical: A0QWG6 (Residues: 1-386; Coverage: 100%)
Gene names: MSMEG_2935, MSMEI_2861, pimA
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 37.071Å b: 72.747Å c: 137.166Å
α: 90° β: 90° γ: 90°
R R work R free
0.196 0.192 0.245
Expression system: Escherichia coli