Structure analysis

Crystal structure of a Novel viral protease with a serine/lysine catalytic dyad mechanism

X-ray diffraction
2.2Å resolution
Source organism: Blotched snakehead virus
Assemblies composition:
monomeric (preferred)
homo 24-mer
homo hexamer
homo dimer
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: monomeric
Accessible surface area: 9892.76 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183832
Assembly 2
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Multimeric state: monomeric
Accessible surface area: 10297.11 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-183832
Assembly 3
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Multimeric state: homo 24-mer
Accessible surface area: 179976.68 Å2
Buried surface area: 62463.73 Å2
Dissociation area: 3,881.92 Å2
Dissociation energy (ΔGdiss): 10.88 kcal/mol
Dissociation entropy (TΔSdiss): 47.73 kcal/mol
Symmetry number: 12
PDBe Complex ID: PDB-CPX-183834
Assembly 4
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Multimeric state: homo hexamer
Accessible surface area: 46940.75 Å2
Buried surface area: 13675.92 Å2
Dissociation area: 2,474.67 Å2
Dissociation energy (ΔGdiss): 3.28 kcal/mol
Dissociation entropy (TΔSdiss): 28.37 kcal/mol
Symmetry number: 6
PDBe Complex ID: PDB-CPX-183835
Assembly 5
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Multimeric state: homo dimer
Accessible surface area: 17246.28 Å2
Buried surface area: 2943.59 Å2
Dissociation area: 1,471.79 Å2
Dissociation energy (ΔGdiss): 11.7 kcal/mol
Dissociation entropy (TΔSdiss): 12.57 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-183833

Macromolecules

Chains: A, B
Length: 217 amino acids
Theoretical weight: 23.66 KDa
Source organism: Blotched snakehead virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8AZM0 (Residues: 558-773; Coverage: 20%)
Pfam: Birnavirus VP4 protein
InterPro: Birnavirus VP4 protease domain
CATH:

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