2gc3

X-ray diffraction
2.1Å resolution

The crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with mannose 6-phosphate and zinc

Released:

Function and Biology Details

Reaction catalysed:
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.38 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P83194 (Residues: 1-187; Coverage: 99%)
Gene names: PF0196, pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 70.346Å b: 74.016Å c: 74.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.158 0.235
Expression system: Escherichia coli BL21