X-ray diffraction
1.25Å resolution

Crystal structure of Thiopurine methyltransferase (18204406) from Mus musculus at 1.35 A resolution

Source organism: Mus musculus
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Thiopurine S-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 29.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: O55060 (Residues: 1-240; Coverage: 100%)
Gene name: Tpmt
Sequence domains: Thiopurine S-methyltransferase (TPMT)
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

Cofactor: Ligand SAH 2 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 62.988Å b: 70.554Å c: 72.593Å
α: 90° β: 115.7° γ: 90°
R R work R free
0.139 not available 0.193
Expression system: Escherichia coli