X-ray diffraction
2Å resolution

X-ray crystal structure of murine leukocyte-type Core 2 b1,6-N-acetylglucosaminyltransferase (C2GnT-L)


Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-glucosamine + O(3)-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O(3)-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein]

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 45.32 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
  • Canonical: Q09324 (Residues: 38-428; Coverage: 91%)
Gene name: Gcnt1
Sequence domains: Core-2/I-Branching enzyme

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 126.779Å b: 79.979Å c: 104.971Å
α: 90° β: 107.7° γ: 90°
R R work R free
0.197 0.197 0.221
Expression system: Cricetulus griseus