2g2w

X-ray diffraction
1.8Å resolution

Crystal Structure of the SHV D104K Beta-lactamase/Beta-lactamase inhibitor protein (BLIP) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase SHV-1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.92 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AD64 (Residues: 22-286; Coverage: 100%)
Gene names: bla, shv1
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily
Beta-lactamase inhibitory protein Chain: B
Molecule details ›
Chain: B
Length: 165 amino acids
Theoretical weight: 17.56 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35804 (Residues: 37-201; Coverage: 100%)
Sequence domains: Beta-lactamase inhibitor (BLIP)
Structure domains: Beta-lactamase Inhibitory Protein; Chain:B, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 42.563Å b: 44.825Å c: 62.902Å
α: 78.88° β: 88.94° γ: 62.42°
R-values:
R R work R free
0.182 0.18 0.222
Expression system: Escherichia coli BL21(DE3)